Lab 6: LAB ASSIGNMENTS
PRE-LAB QUIZ
This week’s quiz is based on the mutageneic primers needed for the QuikChange PCR. You will be given a mutation number and the primers that were used to generate the mutation. Based on this information, determine the specific amino acid change for your mutation.
IN-LAB ACTIVITIES
You and your partner will meet in a BBCU breakout room to work through the lab procedure together.
- Work through the lab procedure and the provided Google doc, which ask you to:
- create a protocol for QuikChange mutagenesis
- complete a sequence alignment
- generate a PyMOL figure
- discuss what makes a good hypothesis and critique several sample hypotheses
- write your own hypothesis
- Put the required information into your slides in the shared class Google slides presentation.
- At the designated time, you will join a TA and several other pairs to present and discuss your slides.
- Complete the lab/partner/self evaluation Google form.
- Get a sharable link to the Google doc and submit it to Canvas.