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Primary Navigation Book Contents Navigation Biochemistry 551: Biochemical Methods, Online Version Lab 1: Structural Analysis of HCAII using PyMOL Lab 1: BACKGROUND Lab 1: PROCEDURE Part 1 Lab 1: PROCEDURE Part 2 Lab 1: PROCEDURE Part 3 Lab 1: PROCEDURE Part 4 Lab 1: LAB ASSIGNMENTS Lab 1: MINI LAB REPORT Lab 2: PCR Amplification of HCAII and pETblue2 Lab 2: BACKGROUND Lab 2: PROCEDURE Part 1 Lab 2: PROCEDURE Part 2 Lab 2: LAB ASSIGNMENTS Lab 2: MINI LAB REPORT Lab 3: Lab-at-home: Introduction to Biotechnology Methods Lab 3: BACKGROUND AND PROCEDURE Lab 3: LAB ASSIGNMENTS Lab 3: WRITE UP Lab 4: Gibson assembly of HCAII into pETblue2 vector and transformation of E. coli with the reactions Lab 4: BACKGROUND Lab 4: PROCEDURE Lab 4: LAB ASSIGNMENTS Lab 4: MINI LAB REPORT Lab 6: Mutant Exploration Lab 6: BACKGROUND Lab 6: PROCEDURE Lab 6: LAB ASSIGNMENTS Lab 6: MINI LAB REPORT Lab 5: Screening for pETblue2-HCAII clones Lab 5: BACKGROUND Lab 5: PROCEDURE Lab 5: LAB ASSIGNMENTS Lab 5: MINI LAB REPORT Lab 7: Protein expression and purification in E. coli Lab 7: BACKGROUND Lab 7: PROCEDURE Lab 7: LAB ASSIGNMENTS Lab 7: MINI LAB REPORT Lab 8: Analysis of His-tagged HCAII expression and purification Lab 8: BACKGROUND Lab 8: PROCEDURE Lab 8: LAB ASSIGNMENTS Lab 8: DATA ANALYSIS Lab 8: MINI LAB REPORT Lab 9: Determination of Protein Stability using Chemical Denaturation and Intrinsic Fluorescence Lab 9: AT-HOME EXPERIMENT Lab 9: BACKGROUND Lab 9: PROCEDURE Lab 9: LAB ASSIGNMENTS Lab 9: DATA ANALYSIS Lab 9 MINI LAB REPORT Lab 10: Investigation of the wild type and mutant HCAII enzyme activity Lab 10: AT-HOME EXPERIMENT Lab 10: BACKGROUND Lab 10: PROCEDURE Lab 10: LAB ASSIGNMENTS Lab 10: DATA ANALYSIS Lab 10: MINI LAB REPORT Lab 11: FRET to detect ligand binding to HCAII Lab 11: BACKGROUND Lab 11: PROCOTOL Lab 11: LAB ASSIGNMENTS Lab 11: DATA ANALYSIS Lab 11: FINAL LAB REPORT Appendix 1: Information on HCAII Appendix 2: Statistical methods used in 551 data analyses Appendix 3: Additional laboratory details Appendix 4: Buffer formulations Appendix 5: Operation of the Shimadzu spectrophotometers Appendix Lab 11 Objectives Previous/next navigation Biochemistry 551 (Online Version) Lab Manual Copyright © by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.