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You can use get_defined_functions() before and after you include the file, and look at what gets added to the array the second time. Since they appear to be in order of definition, you can just use the index like this:
$functions = get_defined_functions();
$last_index = array_pop(array_keys($functions['user']));
// Include your file here.
$functions = get_defined_functions();
$new_functions = array_slice($functions['user'], $last_index);
do include to the file and try this :
$functions = get_defined_functions();
print_r($functions['user']);