I'm a relatively new C programmer, and I've noticed that many conventions from other higher-level OOP languages don't exactly hold true on C.
Is it okay to use short functions to have your coding stay organized (even though it will likely be called only once)? An example of this would be 10-15 lines in something like void init_file(void), then calling it first in main().
Yes. Please. Don't write long functions. Write short ones that do one thing and do it well. The fact that they may only be called once is fine. One benefit is that if you name your function well, you can avoid writing comments that will get out of sync with the code over time.
Functions are absolutely necessary to stay organized. You need to first design the problem, and then depending on the different functionality you need to split them into functions. Some segment of code which is used multiple times, probably needs to be written in a function.
I think first thinking about what problem you have in hand, break down the components and for each component try writing a function. When writing the function see if there are some code segment doing the same thing, then break it into a sub function, or if there is a sub module then it is also a candidate for another function. But at some time this breaking job should stop, and it depends on you. Generally, do not make many too big functions and not many too small functions.
When construction the function please consider the design to have high cohesion and low coupling.
EDIT1::
you might want to also consider separate modules. For example if you need to use a stack or queue for some application. Make it separate modules whose functions could be called from other functions. This way you can save re-coding commonly used modules by programming them as a group of functions stored separately.