How to Do Spectrophotometric Analysis
Turn on the spectrophotometer.. Clean the cuvettes or test tubes.. Load the proper volume of the sample into the cuvette.
Steps
1
Turn on the spectrophotometer.
Turn on the spectrophotometer.
2
Clean the cuvettes or test tubes.
Clean the cuvettes or test tubes.
3
Load the proper volume of the sample into the cuvette.
Load the proper volume of the sample into the cuvette.
4
Prepare a control solution.
Prepare a control solution.
5
Wipe the outside of the cuvette.
Wipe the outside of the cuvette.
6
Choose and set the wavelength of light to analyze the sample with.
Choose and set the wavelength of light to analyze the sample with.
7
Calibrate the machine with the blank.
Calibrate the machine with the blank.
8
Remove the blank and test the calibration.
Remove the blank and test the calibration.
9
Measure the absorbance of your experimental sample.
Measure the absorbance of your experimental sample.
10
Repeat the test with successive wavelengths of light.
Repeat the test with successive wavelengths of light.
11
Calculate the transmittance and absorbance of the sample.
Calculate the transmittance and absorbance of the sample.
12
Plot the absorbance values versus the wavelengths on a graph.
Plot the absorbance values versus the wavelengths on a graph.
13
Compare your absorbance spectrum plot to known plots of specific compounds.
Compare your absorbance spectrum plot to known plots of specific compounds.
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