How to Do Pipette Calibration
Gather the necessary materials.. Measure the temperature of the distilled water.. Place the weigh boat on the balance and zero it out.
Steps
1
Gather the necessary materials.
Gather the necessary materials.
2
Measure the temperature of the distilled water.
Measure the temperature of the distilled water.
3
Place the weigh boat on the balance and zero it out.
Place the weigh boat on the balance and zero it out.
4
Prepare the pipette for calibration.
Prepare the pipette for calibration.
5
Pre-rinse the tip before calibration.
Pre-rinse the tip before calibration.
6
Aspirate the calibration volume.
Aspirate the calibration volume.
7
Dispense the liquid into the weigh boat on the scale.
Dispense the liquid into the weigh boat on the scale.
8
Record the weight on the balance.
Record the weight on the balance.
9
Repeat this process to take at least 10 readings.
Repeat this process to take at least 10 readings.
10
Define the formula for calculated volume.
Define the formula for calculated volume.
11
Average the replicates together.
Average the replicates together.
12
Plug the variables into the equation and solve.
Plug the variables into the equation and solve.
13
Calculate the accuracy of the pipette.
Calculate the accuracy of the pipette.
14
Send the pipette for calibration, if necessary.
Send the pipette for calibration, if necessary.
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